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A karyotypic study of three southern Brazilian Asteraceae species using fluorescence in situ hybridization with a 45S rDNA probe and C-CMA3 banding Genet. Mol. Biol.
Fregonezi,Jéferson N.; Torezan,José M.D.; Vanzela,André L.L..
The Asteraceae, one of the largest families of flowering plants, contains about 1,100 genera and 20,000 species, and is well known for its extensive karyotypic variation. In this study, conventional Feulgen staining, C-CMA3 banding, and fluorescence in situ hybridization with a 45S rDNA probe were used to determine the chromosome number and the number and physical position of GC-rich heterochromatin and 45S rDNA sites in three Asteraceae weed species (Crepis japonica, Galinsoga parviflora and Chaptalia nutans). The three species exhibited karyotype differences in the chromosome number and shape, as a commom feature of Asteraceae. However, the 45S rDNA sites always occurred on the short chromosomal arms, associated with GC-heterochromatin. Althought of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CMA3; FISH; Heterochromatin; Polyploidy; 45S rDNA.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200016
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Chromosomal analyses in Megalonema platanum (Siluriformes: Pimelodidae), an endangered species from South American rivers Neotropical Ichthyology
Carvalho,Rafael Augusto de; Sanchez,Sebástian; Swarça,Ana Claudia; Fenocchio,Alberto Sergio; Martins-Santos,Isabel C.; Dias,Ana Lúcia.
This study presents chromosomal data of Megalonema platanum from rio Tibagi, Paraná, Brazil and from rio Paraná, Argentina. The diploid number was equal 54 with karyotype composition of 24m+16sm+2st+12a in both populations. The AgNOR sites were detected in the terminal position of a submetacentric pair of the two analyzed populations, coinciding with secondary constrictions on the short arm of pair 15. CMA3 and FISH with 18S rDNA probe displayed fluorescent signals that correspond to the AgNOR sites and secondary constriction. The presence of a small acrocentric supernumerary chromosome can be observed in M. platanum from rio Tibagi, with centromeric heterochromatin. Others heterochromatic blocks were evidenced in the terminal position of some chromosome...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; C-banding; CMA3; FISH; NORs; Pimelodidae.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100017
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Cytogenetic characterization of a population of Bryconamericus aff. iheringii (Characidae,Tetragonopterinae) Genet. Mol. Biol.
Paintner-Marques,Tânia Regina; Giuliano-Caetano,Lucia; Dias,Ana Lúcia.
A cytogenetic analysis of thirteen specimens of Bryconamericus aff. Iheringii revealed a diploid number of 52 chromosomes and a karyotype of 8M+22SM+10ST+12A with a fundamental number (FN) of 92. The nucleolus organizer regions (NORs) were studied by means of AgNO3 and CMA3 staining as well as by FISH using an 18S rDNA probe. NORs were found to be located at the terminal position on the short arm of the submetacentric chromosome pair. C-banding showed strong telomeric and centromeric staining in the majority of the chromosomes, and a similar pattern was observed after treatment with AluI restriction enzyme
Tipo: Info:eu-repo/semantics/article Palavras-chave: AgNOR; Alu I; Bryconamericus; C-banding; CMA3; RDNA 18S.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200007
Registros recuperados: 3
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